INTRODUCTION 7
1 LITERATURE REVIEW 8
1.1 General characteristics of Rhodamine 6G 8
1.1.1 Synthesis of Rhodamine 6G 10
1.1.2 Metabolism of Rhodamine 6G 10
1.1.3 Use of Rhodamine 6G 12
1.2 Extraction Method of Rhodamine 6G 13
1.2.1 Solid phase extraction 13
1.3 Analytical Method 16
1.3.1 Spectrophotometry 16
1.3.2 Smartphone-Based Digital Image 17
1.3.2.1Apparatus and Software 20
1.3.2.2 Test Zone Fabrication 20
1.3.2.3 Image Capturing and Analytical Procedures 21
1.3.2.4 Image Capturing Conditions 22
1.3.2.5 Selection of Color Space Model for Image Analysis 23
2 EXPERIMENTAL SECTION 24
2.1 Reagents, Starting Materials and Instruments 24
2.2 Experimental Methods 25
2.2.1 To study of the absorption spectra of rhodamine solutions at different concentrations 25
2.2.2 The effect of pH solution acidity on the analyzed signals 26
2.2.3 To study the effect of the contact time of the solution with the PMM .... 27
2.2.4 Determination of rhodamine in oil field water samples 29
3 RESULTS AND DISCUSSION 33
3. 1 Absorption and luminescence spectra in a polymethacrylate matrix 33
3. 2 Effect of pH of Rhodamine 6G dye solution on its extraction PMM 34
3. 3 Calibration relationship for rhodamine determination using PMM 39
3. 4 Effect of contact time between PMM and dye solution on dye extraction 41
3. 5 Determination of rhodamine in a real object 45
CONCLUSIONS 49
LIST OF ABBREVIATIONS AND SYMBOLS 50
LIST OF REFERENCES 51
... отсутствуют 2 и 3 разделы
Rhodamine 6G (R6G), an organic dye with high fluorescence quantum yield, finds wide application in bioimaging, environmental monitoring, and analytical chemistry. However, concerns regarding its potential toxicity and illegal abuse have led to an urgent need for the development of sensitive and efficient detection methods. In this study, a rapid, cost-effective, and highly sensitive detection system for R6G was developed. This system was developed by optimizing solid phase extraction (SPE) and smartphone digital image analysis. Polymethacrylate matrix (PMM) was utilized as the solid-phase adsorbent material, and the effects of solution pH, contact time, and concentration on the adsorption efficiency of R6G were systematically investigated. This study innovatively combined solid-phase extraction with smartphone image analysis technology, which not only provides a new strategy for the accurate detection of trace R6G, but also provides an efficient technical support for food safety regulation, environmental pollutant monitoring and screening of illegal additives. The method exhibits several advantages, including ease of operation, high sensitivity, and low cost. Furthermore, its capacity for simultaneous multi-dye detection is promising and can be further expanded in the future through the functionalization and modification of PMM materials.
